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中国癌症防治杂志

中国癌症防治杂志 ›› 2011, Vol. 3 ›› Issue (2): 114-.doi: 10.3969/j.issn.1674-5671.2011.02.05

• 基础研究 • 上一篇    下一篇

肿瘤患者外周血有核细胞对化疗药物的敏感性

张 露, 唐东平, 左传田, 张洁清, 唐 凯, 胡晓桦, 张传珉   

  • 出版日期:2011-08-03 发布日期:2011-08-03
  • 基金资助:

    广西自然科学基金(桂科基0575091)；广西科学研究与技术开发计划资助项目(桂科攻0235024-13)

ZHANG Lu, TANG Dong-Ping, ZUO Chuan-Tian, ZHANG Jie-Qing, TANG Kai, HU Xiao-Hua, ZHANG Chuan-Min   

  • Online:2011-08-03 Published:2011-08-03

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摘要： 【摘要】目的 研究恶性肿瘤患者外周血细胞对化疗药物的敏感性，为临床筛选化疗方案、实行肿瘤个体化疗提供依据。方法 应用MTT法体外测定160例癌患者外周血(淋巴细胞及粒细胞)标本对临床常用化疗药物的敏感性。结果 ①5种癌患者外周血淋巴细胞和粒细胞对同一化疗药物敏感率呈正相关性，且均有统计学意义(P＜0.05)。②胃癌、非小细胞肺癌(NSCLC)和卵巢癌患者的外周血淋巴细胞及粒细胞对试验化疗药物敏感性差异有统计学意义(P＜0.05)，而肝癌、淋巴瘤(NHL)检测结果差异未有统计学意义(P＞0.05)。③HCPT在非小细胞肺癌和TAX、Vm-26在卵巢癌患者的外周血药敏试验中，粒细胞较淋巴细胞对化疗药物抑制率高，差异有统计学意义(P＜0.05)。结论肿瘤患者外周血细胞体外药敏试验对临床肿瘤化疗用药有一定预见性，有助于临床医师筛选有效化疗药物，制定合理化疗方案，为个体化疗用药提供了重要的参考依据。

关键词: 【关键词】消化系肿瘤, 非小细胞肺癌, 卵巢癌, 淋巴瘤, 外周血有核细胞, MTT法, 化疗药敏试验

Abstract: 【Abstract】Objective To study the chemosensitivity to anticancer drugs of peripheral blood lymphocytes and granulocytes collected from cancer patients，and then to choose chemotherapeutics for the patients individually. Methods The sensitivity of the commonly used chemotherapeutic drugs in the peripheral blood lymphocytes and granulocytes collected from 160 patients with 5 kinds of cancer were tested by MTT assay in vitro. Results The sensitivities of lymphocytes and granulocytes collected from five kind of cancer patients were all positively correlated (P＜0.05). The inhibition rations of the anticancer drugs on peripheral blood nucleated cells collected from the patients with stomach cancer，non-small-cell lung cancer (NSCLC) and ovarian cancer were statistically different (P＜0.05)，while from the patients with liver cancer and NHL was not significantly different. The resistance of granulocytes was higher than that of lymphocytes in the tests of HCPT on NSCLC，TAX and Vm-26 on ovarian cancer. Conclusion Chemotherapy under the guidance of chemosensitivity test in vitro with peripheral blood nucleated cells may improve the therapeutic effect and help clinician to choose reasonable chemotherapy drugs for patients individually.

Key words: 【Key words】 Digestive system cancer, Non-small-cell lung cancer, Ovarian cancer, NHL, Peripheral blood nucleated cells, MTT assay, Chemosensitivity test